| Form |
white to off-white fine crystalline powder |
| MF |
C27H44O |
| MW | 384.64 |
| Melting point |
148-152 °C(lit.) |
| Boiling point |
451.27°C (rough estimate) |
| Density |
0.9717 (rough estimate) |
| Refractive index |
1.5100 (estimate) |
| Pka |
14.91±0.70(Predicted) |
| Storage temp. | -20°C |
|
ITEM |
STANDARD |
|
APPEARANCE |
WHITE OR OFF-WHITE FLOWING POWDER |
|
SOLUBILITY |
EASILY DISPERSED IN COLD WATER 15℃ TO FORM A HOMOGENEOUS AND STABLE EMUSION |
|
GRANULARITY:GO THROUGH THE SIEVE OF 60 MESH |
>=90.0% |
|
HEAVY METAL |
=<10PPM |
|
LEAD |
=<2PPM |
|
ARSENIC |
=<1PPM |
|
MERCURY |
=<0.1PPM |
|
CADMIUM |
=<1PPM |
|
LOSS ON DRYING |
NOT MORE THAN 5.0% |
|
Vitamin D3 Content |
>=500,000IU/g |
|
TOTAL PLATE COUNT |
=<1000CFU/G |
|
YEAST&MOULD |
=<100CFU/G |
|
COLIFORMS |
=<0.3MPN/G |
|
E.COLI |
NEGATIVE/10G |
|
SALMONELLA |
NEGATIVE/25G |
Vitamin D3 is produced by the action of sunlight or ultraviolet irradiation from the precursor, 7-dehydrocholesterol (7-DC), that is synthesized in the skin of animals and humans.
In most tissues of the body, 7-dehydrocholesterol is the immediate precursor of cholesterol. It is reduced to cholesterol by the enzyme 3β-hydroxysterol-Δ7-reductase (DHCR7) in the last step of cholesterol biosynthesis. 7-DHC accumulates in Smith-Lemli-Opitz syndrome (SLOS), a disorder characterized by a mutation in the DHCR7 gene and decreased cholesterol levels in bodily tissues and fluids, as well as microcephaly, intellectual disability, and distinctive dysmorphic features. It is highly susceptible to free radical oxidation, giving rise to several oxysterols that may be involved in the pathogenesis of SLOS. 7-DHC levels are increased in brain, liver, and serum in a rat model of SLOS induced by the DHCR7 inhibitor AY 9944. 7-DHC is a provitamin that is converted to vitamin D3 by ultraviolet-B (UVB) light in a human skin equivalent system and in isolated human skin samples.
